Refereed Publications from Peking University Shenzhen Graduate School (July/2016-present):[#Co-first authors; *Corresponding authors]
(9) Liu M#, Li J#, Liu W#, Yang Y#, Zhang M, Ye Y, Zhu W, Zhou C, Zhai H, Xu Z*, Zhang G*, Huang H*.
The S1'-S3' Pocket of the SARS-CoV-2 Main Protease Is Critical for Substrate Selectivity and Can Be Targeted with Covalent Inhibitors, Angew Chem Int Ed, 2023: doi: 10.1002/anie.202309657.
(8) Xiong Y#, Huang B#, Yang Y, Fu X, Fu Z, Xu H, Liu M, Cao D, Zhang M, Yang H, Niu X, Yu C, Huang H*,
Papain-like proteases from seven human-infecting coronaviruses differ in substrate selectivity and innate immune suppression, Sci. Signal., 2023,16(783):eade1985.
(7) Cao D#, Duan L#, Huang B#, Xiong Y, Zhang G*, Huang H*,
The SARS-CoV-2 papain-like protease suppresses type I interferon responses by deubiquitinating STING, Sci. Signal., 2023,16(783):eadd0082.
(6) Tang J#, D
Identification of Small Molecule Inhibitors of RNase L by Fragment-Based Drug Discovery, J Med Chem. 2022 Jan 27;65(2):1445-1457.
(5)
The complex structure of GRL0617 and SARS-CoV-2 PLpro reveals a hot spot for antiviral drug discovery, Nat Commun. 2021 Jan 20;12(1):488.
(4) Ye Y#, Xiong Y#, Huang H*.
Substrate-binding destabilizes the hydrophobic cluster to relieve the autoinhibition of bacterial ubiquitin ligase IpaH9.8, Commun Biol. 2020 Dec 10;3(1):752.
(3) Tang J#, Wang Y#, Zhou H#, Ye Y, Talukdar M, Fu Z, Liu Z, Li J, Neculai D, Gao J, Huang H*,
Sunitinib inhibits RNase L by destabilizing its active dimer conformation , Biochem J . 2020 Sep 18;477(17):3387-3399.
(2) Daou S#, Talukdar M#, Tang J#, Dong B, Banerjee S, Li Y, Duffy NM, Ogunjimi AA, Gaughan C, Jha BK, Gish G, Tavernier N, Mao D, Weiss SR, Huang H*, Silverman RH*, Sicheri F*,
A phenolic small molecule inhibitor of RNase L prevents cell death from ADAR1 deficiency, Proc Natl Acad Sci U S A. 2020 Oct 6;117(40):24802-24812.
(1) Wan LC, Maisonneuve P, Szilard RK, Lambert JP, Ng TF, Manczyk N, Huang H, Laister R, Caudy AA, Gingras AC, Durocher D, Sicheri F,
Proteomic analysis of the human KEOPS complex identifies C14ORF142 as a core subunit homologous to yeast Gon7, Nucleic Acids Res. 2017, 45(2):805-817.
Refereed Publications from Mount Sinai Hospital/University of Toronto (Mar/2011-Mar/2015):
(4) Huang H, Zeqiraj E, Dong B, Jha BK, Duffy NM, Orlicky S, Thevakumaran N, Talukdar M, Pillon MC, Ceccarelli DF, Wan LC, Juang YC, Mao DY, Gaughan C, Brinton MA, Perelygin AA, Kourinov I, Guarné A, Silverman RH, Sicheri F, Dimeric structure of pseudokinase RNase L bound to 2-5A reveals a basis for interferon induced antiviral activity. Mol Cell. 2014; 53, 221-34
Recommended by F1000: http://f1000.com/prime/718246713
Highlighted in Cell 157, 2014: http://www.cell.com/cell/pdf/S0092-8674(14)00487-5.pdf
(3) Huang H*, Ceccarelli DF*, Orlicky S*, St-Cyr DJ, Ziemba A, Garg P, Plamondon S, Auer M, Sidhu S, Marinier A, Kleiger G, Tyers M, Sicheri F, E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. Nat Chem Biol. 2014 10, 156-63
[*Co-first authors]
(2) Fradet-Turcotte A, Canny MD, Escribano-Díaz C, Orthwein A, Leung CC, Huang H, Landry MC, Kitevski-LeBlanc J, Noordermeer SM, Sicheri F, Durocher D. 53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark. Nature. 2013, 499, 50-54.
(1) Rivkin E, Almeida SM, Ceccarelli DF, Juang YC, MacLean TA, Srikumar T, Huang H, Dunham WH, Fukumura R, Xie G, Gondo Y, Raught B, Gingras AC, Sicheri F, Cordes SP. The linear ubiquitin-specific deubiquitinase gumby regulates angiogenesis. Nature. 2013, 498, 318-24
Refereed publications from the University of Calgary (Jan/2006-Jan/2011):
(5) Huang H, Bogstie JN, Vogel HJ, Biophysical and Structural Studies of the Human Calcium- and Integrin-Binding Protein Family: Understanding their Functional Similarities and Differences. Biochem. Cell Biol., 2012, 90, 646-56
(4) Huang H and Vogel HJ, Structural Basis for the Activation of the Cytoplasmic Domain of the Human Platelet Integrin AlphaIIb-beta3 by CIB1. J Am Chem Soc., 2012, 134, 3864-72
Highlighted in Science Daily: http://www.sciencedaily.com/releases/2012/03/120319095011.htm
(3) Huang H, Ishida H, Yamniuk, AP, Vogel HJ, The Solution Structures of Ca2+- CIB1 and Mg2+-CIB1 and their interactions with the Platelet Integrin αIIb Cytoplasmic Domain. J Biol Chem., 2011, 286, 17181-92
(2) Huang H, Ishida H, Vogel HJ, The Solution Structure of the Mg2+- form of Soybean Calmodulin Isoform 4 Reveals Unique Features of Plant Calmodulins in Resting Cells. Protein Sci., 2010, 19, 475-85
(1) Ishida H, Huang H, Yamniuk AP, Takaya Y, Vogel HJ, The solution structures of two soybean calmodulin isoforms provide a structural basis for their selective target-activation properties. J Biol Chem., 2008, 283,14619-28
Non-Refereed Publication (Book Chapter) from the University of Calgary (Jan/2006-Jan/2011):
(1) Huang H and Vogel HJ, Purification and Stable Isotope Labeling of the Calcium and Integrin Binding Protein 1 for Structural and Functional NMR Studies. Methods Mol Biol. 2013, 963, 99-113
Refereed Publications from McMaster University (Jan/2004-Dec/2005):
(2) Huang H, Milojevic J, Melacini G, Analysis and optimization of saturation transfer difference NMR experiments designed to map early self-association events in amyloidogenic peptides. J Phys Chem B., 2008,112, 5795-802
(1) Huang H, Melacini G. High-resolution protein hydration NMR experiments: Probing how protein surfaces interact with water and other non-covalent ligands. Anal Chim Acta, 2006, 564, 1-9.
Refereed Publications from Tsinghua University (Sep/1999-July/2002):
(3) Huang H, Jing L, Qin Z, Zhou Q, Sun SQ, The application of Fourier transform infrared spectroscopy on the quality control of traditional chinese medicine formula particles. Chinese Journal of Analytical Chemistry, 2003, 31, 828-32. [in Chinese]
(2) Huang H, Sun SQ, Xu JW, Wang Z, Dongxiang Li, Novel application of FTIR to medical herb chemotaxonomy. Spectroscopy and Spectral Analysis, 2003, 23, 253-57. [in Chinese]
(1) Huang H, Xiao X, Gao H, Sun SQ, Feng J, Luo GA, Rapid Detection of Escherichia coli by Electrochemical Assay of β-D-Galactosidase with p-Aminophenyl-β-D-Galactopyranoside as Substrate. Tsinghua Science and Technology, 2002, 7, 624-629. [in English]
Patent
Tyers M, Sicheri F, Ceccarelli DF, Huang H, et al., Screening methods to identify compounds inhibiting the activity of E2 enzymes by stabilization of non-covalent ubiquitin-E2 complexes and pharmaceutical applications related to E2 inhibitors. Application #: PCT/CA2013/001079;