Hong Wang, Master
Email: Wanghong@pkusz.edu.cn
EDUCATION:
Sep, 1999-Jul, 2002 Master, Department of Plant Physiology and Molecular Biology, China Agricultural University, Beijing, China
Sep, 1995-Jul, 1999 Bachelor of Science, Department of Physiology and Biophysics, School of Life Sciences, Peking University, Beijing, China
RESEARCH EXPERIENCE:
Sep, 2006-Sep, 2013 Staff Research Associate, Division of Endocrinology and Metabolism, Department of School of Medicine, University of California at San Diego
Supervisor: Wolfgang Dillmann, Chair, Department of Medicine, UCSD
Sep. 2002-Jul. 2005 Biology Teacher of the High School Attached to Tsinghua University
Dec. 1998-Jul. 2002 Master Candidate, Key Research Laboratory in Plant Physiology and Biochemistry & College of Biological Science, China Agricultural University
Supervisor: Wei-Hua Wu, Academician of Chinese Academy of Sciences
Professional Skills:
- Molecular Biology: DNA, RNA and Protein extraction and purification; PCR; DNA cloning; Real-time PCR; Southern, Northern and Western Blotting
- Cell Biology: Isolation and culture of cardiac myocytes (neonatal or adult; mouse or rat), endothelial and smooth muscle cells; Confocal microscopy, Electron Microscopy, Immunostaining, Single cell microinjection
- Physiology: Measurement of mitochondrial respiration, oxidation and membrane potential; Calcium transient recording; Measurement of cell contractility
- Small Surgery: Mouse jugular and tail vein injection; Gene therapy
- Computer: Excel, Words, Powerpoint, Adobe Photoshop/Image J
PUBLICATIONS:
10. Suarez J, McDonough PM, Scott BT, Suarez-Ramirez A, Wang H, Fricovsky ES, Dillmann WH. (2013) Sorcin modulates mitochondrial Ca(2+) handling and reduces apoptosis in neonatal rat cardiac myocytes. Am J Physiol Cell Physiol. 304(3): C248-56.
9. Gao MH, Lai NC, Tang T, Guo T, Tang R, Chun BJ, Wang H, Dalton NN, Suarez J, Dillmann WH, Hammond HK. (2013) Preserved cardiac function despite marked impairment of cAMP generation. PLoS One. 16;8(9) :e72151. doi: 10.1371/journal.pone.0072151
8. Fricovsky ES, Suarez J, Ihm SH, Scott BT, Suarez-Ramirez JA, Banerjee I, Torres-Gonzalez M, Wang H, Ellrott I, Maya-Ramos L, Villarreal F, Dillmann WH. (2012) Excess protein O-GlcNAcylation and the progression of diabetic cardiomyopathy. Am J Physiol Regul Integr Comp Physiol. 303(7): R689-99.
7. Makino A, Wang H, Scott BT, Yuan JX, Dillmann WH. (2012) Thyroid Hormone Receptor α and Vascular Function. Am J Physiol Cell Physiol. 302(9):C1346-52.
6. Gawlowski T, Suarez J, Scott B, Torres-Gonzalez M, Wang H, Schwappacher R, Han X, Yates JR 3rd, Hoshijima M, Dillmann W. (2012) Modulation of dynamin-related protein 1 (DRP1) function by increased O-linked-β-N-acetylglucosamine modification (O-GlcNAc) in cardiac myocytes. J Biol Chem. 287(35):30024-34.
5. Makino A, Suarez J, Gawlowski T, Han W, Wang H, Scott BT, Dillmann WH (2011) Regulation of Mitochondrial Morphology and Function by O-GlcNAcylation in Neonatal Cardiac Myocytes. Am J Physiol Regul Integr Comp Physiol. 300(6):R1296-302.
4. Makino A, Suarez J, Wang H, Belke DD, Scott BT, Dillmann WH (2009) Thyroid hormone receptor-beta is associated with coronary angiogenesis during pathological cardiac hypertrophy. Endocrinology. 150(4): 2008-15
3. Hu Y, Suarez J, Fricovsky E, Wang H, Scott BT, Trauger SA, Han W, Hu Y, Oyeleye MO, Dillmann WH (2009) Increased enzymatic O- GlcNAcylation of mitochondrial proteins impairs mitochondrial function in cardiac myocytes exposed to high glucose. J Biol Chem. 284(1): 547-55
2. Suarez J, Hu Y, Makino A, Fricovsky E, Wang H, Dillmann WH (2008) Alterations in mitochondrial function and cytosolic calcium induced by hyperglycemia are restored by mitochondrial transcription factor A in cardiomyocytes. Am J Physiol Cell Physiol. 295(6): C1561-8
1. Fan LM, Wang YF, Wang H, Wu WH (2001) In vitro Arabidopsis pollen germination and characterization of the inward potassium currents in Arabidopsis pollen grain protoplasts. J Exp Bot. 52(361): 1603-14.
