Laboratory of Molecular & Cardiovascular Physiology
In the Drug Discovery Center


62. Siting Zhu, Ze’e Chen, Mason Zhu, Ying Shen, Leonardo J. Leon, Liguo Chi, Simone Spinozzi, Changming Tan, Yusu Gu, Anh Nguyen, Yi Zhou, Wei Feng, Frédéric M. Vaz, Xiaohong Wang, Asa B. Gustafsson, Sylvia M. Evans, Ouyang Kunfu#, Xi Fang#. (2021) Cardiolipin Remodeling Defects Impair Mitochondrial Architecture and Function in a Murine Model of Barth Syndrome Cardiomyopathy. Circ Heart Fail. 14(6): e008289. (#co-corresponding author)

61. Li Y, Dong M, Wang Q, Kumar S, Zhang R, Cheng W, Xiang J, Wang G, Ouyang K, Zhou R, Xie Y, Lu Y, Yi J, Duan H, Liu J. (2021) HIMF deletion ameliorates acute myocardial ischemic injury by promoting macrophage transformation to reparative subtype. Basic Res Cardiol. 116(1): 30. doi: 10.1007/s00395-021-00867-7.

60. Wang S, Wu X, Zhang Y, Zhang D, Xie B, Pan Z, Ouyang K, Peng T. (2021) Discovery of a highly efficient nitroaryl group for detection of nitroreductase and imaging of hypoxic tumor cells. Org. Biomol. Chem. 19(15): 3469-3478. doi: 10.1039/d1ob00356a.

59. Li Y, Tang H#, Chen F, Chen J, Wang H, Chen Z, Duan Y, Wang X, Li L#, Ouyang K#. (2021) SETD2 is essential for terminal differentiation of erythroblasts during fetal erythropoiesis. Biochem Biophys Res Commun. 552:98-105. doi: 10.1016/j.bbrc.2021.03.040. 

58. Chen F, Chen J, Wang H, Tang H, Huang L, Wang S, Wang X, Fang F, Liu J, Li L#, Ouyang K#, Han Z#. (2021) Histone lysine methyltransferase SETD2 regulates coronary vascular development in embryonic mouse hearts. Front Cell Dev Biol. 9: 651655.doi: 10.3389/fcell.2021.651655. (#co-corresponding author)

57. Chen Z, Huang L, Tso A, Wang S, Fang X, Ouyang K#, Han Z#. (2021) Mitochondrial chaperones and proteases in cardiomyocytes and heart failure. Front Mol Biosci. 8: 630332. doi: 10.3389/fmolb.2021.630332. (#co-corresponding author; Review)

56. Chen Z, Duan Y, Wang H, Tang H, Wang S, Wang X, Liu J, Fang X#, Ouyang K#. (2021) Atypical protein kinase C is essential for embryonic vascular development in mice. Genesis. e23412. doi: 10.1002/dvg.23412.

55. Zhang P, Huang JJ, Ou-Yang KF, Liang H, Li ML, Wang YJ, Yang HT. (2020). Minimal contribution of IP3R2 in cardiac differentiation and derived ventricular-like myocytes from human embryonic stem cells. Acta Pharmacol Sin. doi: 10.1038/s41401-020-00528-w.

54. Liu C, Spinozzi S, Feng W, Chen Z, Zhang L, Zhu S, Wu T, Fang X, Ouyang K, Evans SM, Chen J. (2020) Homozygous G650del nexilin variant causes cardiomyopathy in mice. JCI Insight. 5(16):138780. doi: 10.1172/jci.insight.138780.

53. Dong Y, Lee Y, Cui K, He M, Wang B, Bhattacharjee S, Zhu B, Yago T, Zhang K, Deng L, Ouyang K, Wen A, Cowan DB, Song K, Yu L, Brophy ML, Liu X, Wylie-Sears J, Wu H, Wong S, Cui G, Kawashima Y, Matsumoto H, Kodera Y, Wojcikiewicz RJH, Srivastava S, Bischoff J, Wang DZ, Ley K, Chen H. (2020) Epsin-mediated degradation of IP3R1 fuels atherosclerosis. Nat Commun. 11(1):3984. doi: 10.1038/s41467-020-17848-4.

52. Spinozzi S, Liu C, Chen Z, Feng W, Zhang L, Ouyang K, Evans SM, Chen J. (2020) Nexilin Is Necessary for Maintaining the Transverse-Axial Tubular System in Adult Cardiomyocytes. Circ Heart Fail. doi: 10.1161/CIRCHEARTFAILURE.120.006935.

51. Quan C, Du Q, Li M, Wang R, Ouyang Q, Su S, Zhu S, Chen Q, Sheng S, Chen L, Wang H, Campbell DG, MacKintosh C, Yang Z, Ouyang K, Wang HY, Chen S. (2020) A PKB-SPEG signaling nexus links insulin resistance with diabetic cardiomyopathy by regulating calcium homeostasis. Nat Commun. 11, 2186 DOI:

50. Duan Y, Tang H, Mitchell-silbaugh K, Fang X, Han Z, Ouyang K. (2020) Heat shock protein 60 in cardiovascular physiology and diseases. Front Mol Biosci. 7: 73. Doi: 10.3389/fmolb.2020.00073. (Review).

49. Yang F, Huang L, Tso A, Wang H, Cui L, Lin L, Wang H, Ren M, Fang X, Liu J, Han Z, Chen J, Ouyang K. (2020) Inositol 1,4,5-trisphosphate receptors are essential for fetal-maternal connection and embryo viability. Plos Genetics. 16(4): e1008739. doi: 10.1371/journal.pgen.1008739.

48. Duan Y, Wang H, Mitchell-silbaugh K, Cai S, Fan F, Li Y, Tang H, Wang G, Fang X, Liu J, Jia N, Jing R, Ouyang K. (2019) Heat shock protein 60 regulates yolk sac erythropoiesis in mice. Cell Death Dis. 10: 766. Doi: 10.1038/s41419-019-2014-2.

47. Pinto-Duarte A, Roberts AJ, Ouyang K, Sejnowski TJ. (2019) Impairments in remote memory caused by the lack of Type 2 IP3 receptors. Glia. DOI: 10.1002/glia.23679. [Epub ahead of print]

46. Fan F, Duan Y, Yang F, Trexler C, Wang H, Huang L, Li Y, Tang H, Wang G, Fang X, Liu J, Jia N, Chen J, Ouyang K. (2019) Deletion of heat shock protein 60 in adult mouse cardiomyocytes perturbs mitochondrial protein homeostasis and causes heart failure. Cell Death Diff. doi: 10.1038/s41418-019-0374-x. [Epub ahead of print]

45. Liu C, Spinozzi S, Chen JY, Fang X, Feng W, Perkins G, Cattaneo P, Guimarães-Camboa N, Dalton ND, Peterson KL, Wu T, Ouyang K, Fu XD, Evans SM, Chen J. (2019) Nexilin is a New Component of Junctional Membrane Complexes Required for Cardiac T-Tubule Formation. Circulation. doi: 10.1161/CIRCULATIONAHA.119.039751. [Epub ahead of print]

44. Zhang Y, Ouyang K, Lipina TV, Wang H, Zhou Q. (2019) Conditioned stimulus presentations alter anxiety level in fear-conditioned mice. Mol Brain. 12(1): 28. doi: 10.1186/s13041-019-0445-4.

43. Kumar S, Wang G, Zheng N, Cheng W, Ouyang K, Lin H, Liao Y, Liu J. (2019) HIMF (Hypoxia-Induced Mitogenic Factor)-IL (Interleukin)-6 Signaling Mediates Cardiomyocyte-Fibroblast Crosstalk to Promote Cardiac Hypertrophy and Fibrosis. Hypertension. 73(5):1058-1070. doi: 10.1161/HYPERTENSIONAHA. 118.12267. [Epub ahead of print]

42. Lin Q, Zhao L, Jing R, Trexler C, Wang H, Li Y, Tang H, Huang F, Zhang F, Fang X, Liu J, Jia N, Chen J, Ouyang K. (2019) IP3Rs in endothelial cells play an essential role in vasodilation and blood pressure regulation. J Am Heart Assoc. 8(4): e011704. doi: 10.1161/JAHA.118.011704.

41. Quan C, Li M, Du Q, Chen Q, Wang H, Campbell DG, Fang L, Xue B, MacKintosh C, Gao X, Ouyang K, Wang HY, Chen S. (2019) SPEG Controls Calcium Re-Uptake into the Sarcoplasmic Reticulum Through Regulating SERCA2a by Its Second Kinase-Domain. Circ Res. 124(5): 712-726. 

40. Fang X, Bogomolovas J, Zhou P, Mu Y, Ma X, Chen Z, Zhang L, Zhu M, Veevers J, Ouyang K, Chen J. (2019) P209L Mutation in BAG3 Does Not Cause Cardiomyopathy in Mice. Am J Physiol Heart Circ Physiol. 316(2): H392-H399. doi: 10.1152/ajpheart.00714.2018.

39. Wang H, Jing R, Trexler C, Li Y, Tang H, Pan Z, Zhu S, Zhao B, Fang X, Liu J, Chen J, Ouyang K. (2019) Deletion of IP3R1 by Pdgfrb-Cre in mice results in intestinal pseudo-obstruction and lethality. J Gastroenterol. 54(5):407-418. doi: 10.1007/s00535-018-1522-7.

38. Veevers J, Farah EN, Corselli M, Witty AD, Palomares K, Vidal JG, Emre N, Carson CT, Ouyang K, Liu C, van Vliet P, Zhu M, Hegarty JM, Deacon DC, Grinstein JD, Dirschinger RJ, Frazer KA, Adler ED, Knowlton KU, Chi NC, Martin JC, Chen J, Evans SM. (2018) Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells. Stem Cell Reports. 11: 1-14; doi: 10.1016/j.stemcr.2018.07.007.

37. Xiao Y, Wang C, Chen JY, Lu F, Wang J, Hou N, Hu X, Zeng F, Ma D, Sun X, Ding Y, Zhang Y, Zheng W, Liu Y, Shang H, Zhu W, Han C, Zhang Y, Ouyang K, Chen L, Chen J, Xiao RP, Li CY, Zhang X. (2018) Deficiency of PRKD2 triggers hyperinsulinemia and metabolic disorders. Nat Commun. 9(1): 2015. doi: 10.1038/s41467-018-04352-z.

36. Fang X, Bogomolovas J, Wu T, Zhang W, Liu C, Veevers J, Stroud MJ, Zhang Z, Ma X, Mu Y, Lao DH, Dalton ND, Gu Y, Wang C, Wang M, Liang Y, Lange S, Ouyang K, Peterson KL, Evans SM, Chen J. (2017). Loss-of-function mutations in co-chaperone BAG3 destabilize small HSPs and cause cardiomyopathy. J Clin Invest. 127(8): 3189-3200.

35. Tang H, Wang H, Lin Q, Fan F, Zhang F, Peng X, Fang X, Liu J, Ouyang K#. (2017) Loss of IP3 Receptor–Mediated Ca2+ Release in Mouse B Cells Results in Abnormal B Cell Development and Function. J Immunol. pii: ji1700109. doi: 10.4049/jimmunol.1700109. [Epub ahead of print]

34. Wang YJ, Huang J, Liu W, Kou X, Tang H, Wang H, Gao S, Ouyang K, Yang H-T. (2017) IP3R-Mediated Ca2+ Signals Govern Hematopoietic and Cardiac Divergence of Flk1+ Cells via the Calcineurin-NFATc3-Etv2 Pathway. J Mol Cell Biol. doi: 10.1093/jmcb/mjx014. [Epub ahead of print]

33. Qingsong Lin, Guiling Zhao, Xi Fang, Xiaohong Peng, Huayuan Tang, Hong Wang, Ran Jing, Jie Liu, W. Jonathan Lederer, Ju Chen#, Kunfu Ouyang#. (2016) IP3 receptors regulate vascular smooth muscle contractility and hypertension. JCI insight. 1(17):e89402. doi: 10.1172/jci.insight. 89402. (*, Co-correponding author)

32. Fang X, Stroud MJ, Ouyang K, Fang L, Zhang J, Dalton ND, Gu Y, Wu T, Peterson KL, Huang HD, Chen J, Wang N. (2016) Adipocyte-specific loss of PPARγ attenuates cardiac hypertrophy. JCI Insight. 1(16):e89908. doi:10.1172/jci.insight.89908.

31. Huang J, Zhang M, Zhang P, Liang H, Ouyang K, Yang HT. (2016) Coupling switch of P2Y-IP3 receptors mediates differential Ca2+ signaling in human embryonic stem cells and derived cardiovascular progenitor cells. Purinergic Signal. 12(3):465-78

30. Wei C, Qiu J, Zhou Y, Xue Y, Hu J, Ouyang K, Banerjee I, Zhang C, Chen B, Li H, Chen J, Song LS, Fu XD. (2015) Repression of the Central Splicing Regulator RBFox2 Is Functionally Linked to Pressure Overload-Induced Heart Failure. Cell Rep. pii: S2211-1247(15)00141-2. doi: 10.1016/j.celrep.2015.02.013. [Epub ahead of print]

29. Sherin I. Hashem, Cynthia N. Perry, Matthieu Bauer, Sangyoon Han, Stacey D. Clegg, Kunfu Ouyang, Dekker C. Deacon, Mary Spinharney, Athanasia D. Panopoulos, Juan C. Belmonte, Kelly A. Frazer, Ju Chen, Qiuming Gong, Zhengfeng Zhou, Neil C. Chi, and Eric D. Adler. (2015) Oxidative Stress Mediates Cardiomyocyte Apoptosis in a Human Model of Danon Disease and Heart Failure. Stem Cells. doi: 10.1002/stem.2015. [Epub ahead of print]

28. Yongxin Mu, Ran Jing, Angela K. Peter, Stephan Lange, Lizhu Lin, Jianlin Zhang, Kunfu Ouyang, Xi Fang, Jennifer Veevers, Xinmin Zhou, Sylvia M. Evans, Hongqiang Cheng, Ju Chen. (2015) Cypher and Enigma Homolog Protein Are Essential for Cardiac Development and Embryonic Survival. J Am Heart Assoc 4:e001950 doi: 10.1161/JAHA.115.001950.

27. Zhang Z, Stroud MJ, Zhang J, Fang X, Ouyang K, Kimura K, Mu Y, Dalton ND, Gu Y, Bradford WH, Peterson KL, Cheng H, Zhou X, Chen J. (2015) Normalization of Naxos plakoglobin levels restores cardiac function in mice. J. Clin. Invest. 125(4):1708–1712. doi:10.1172/JCI80335. 

26. Chapman MA, Zhang J, Banerjee I, Guo LT, Zhang Z, Shelton GD, Ouyang K, Lieber RL, Chen J. (2014) Disruption of both nesprin 1 and desmin results in nuclear anchorage defects and fibrosis in skeletal muscle. Hum Mol Genet. 23(22): 5879-92. Download this article

25. Kunfu Ouyang*, Rafael Gomez-Amaro*, David Stachura*, Huayuan Tang, Xiaohong Peng, Xi Fang, David Traver, Sylvia M Evans, Ju Chen. (2014) Loss of IP3R-mediated Ca2+ signaling in thymocytes leads to aberrant development and acute lymphoblastic leukemia. Nat. Commun. doi: 10.1038/ncomm5814. (*, Co-first author). Download this article

24. Banerjee I, Zhang J, Moore-Morris T, Pfeiffer E, Buchholz KS, Liu A, Ouyang K, Stroud MJ, Gerace L, Evans SM, McCulloch A, Chen J. (2014) Targeted ablation of nesprin 1 and nesprin 2 from murine myocardium results in cardiomyopathy, altered nuclear morphology and inhibition of the biomechanical gene response. PLoS Genet. 10(2):e1004114. Download this article

23. Lyon RC, Mezzano V, Wright AT, Pfeiffer E, Chuang J, Banares K, Castaneda A, Ouyang K, Cui L, Contu R, Gu Y, Evans SM, Omens JH, Peterson KL, McCulloch AD, Sheikh F. (2014) Connexin defects underlie arrhythmogenic right ventricular cardiomyopathy in a novel mouse model. Hum Mol Genet. 23(5):1134-50. Download this article

22. Xiang SY, Ouyang K, Yung BS, Miyamoto S, Smrcka AV, Chen J, Brown JH. (2013) PLCe, PKD1, and SSH1L Transduce RhoA Signaling to Protect Mitochondria from Oxidative Stress in the Heart. Sci Signal. 6 (306): 1-11. Download this article

21. Lin C, Guo X, Lange S, Liu J, Ouyang K, Yin X, Jiang L, Cai Y, Mu Y, Sheikh F, Ye S, Chen J, Ke Y, Cheng H.(2013) Cypher/ZASP is a novel A kinase anchoring protein. J Biol Chem. 288 (41): 29403–29413. Download this article

20. Ruilin Zhang, Peidong Han, Hongbo Yang, Kunfu Ouyang, Derek Lee, Yi-Fan Lin, Karen Ocorr, Guson Kang, Ju Chen, Didier Y.R. Stainier, Deborah Yelon, Neil C. Chi (2013) In Vivo Cardiac Reprogramming Contributes to Zebrafish Heart Regeneration. Nature 498: 497–501. Download this article

19. Nicola Cooley*, Kunfu Ouyang*, Julie R. McMullen, Junichi Sadoshima, Helen Kiriasis, Wei Wu, Yongxin Mu, Xiao-Jun Du, Ju Chen and Elizabeth A. Woodcock (2013) No contribution of IP3-R(2) to disease phenotype in models of dilated cardiomyopathy or pressure overload hypertrophy. Circulation: Heart Failure 6: 318-325. (*, Co-first author). Download this article

18. Yuanchao Xue, Kunfu Ouyang, Jie Huang, Yu Zhou, Hong Ouyang, Hairi Li, Gang Wang, Qijia Wu, Chaoliang Wei, Yanzhen Bi, Li Jiang, Zhiqiang Cai, Hui Sun, Kang Zhang, Yi Zhang, Ju Chen and Xiang-Dong Fu (2013) Direct Conversion of Fibroblasts to Neurons by Reprogramming PTB-Regulated microRNA Circuits. Cell 152: 82–96. Download this article

17. Wang Y, Li G, Goode J, Paz JC, Ouyang K, Screaton R, Fischer WH, Chen J, Tabas I, Montminy M. (2012) InsP3 receptor regulates hepatic gluconeogenesis in fasting and diabetes. Nature 485: 128-132. Download this article

16. Sheikh F*, Ouyang K*, Campbell S*, Lyon R, Chuang J, Fitzsimons D, Tangney J, Hidalgo C, Chung C, Cheng H, Dalton ND, Gu Y, Kasahara H, Ghassemian M, Omens J, Peterson K, Granzier H, Moss R, McCulloch AD and Chen J (2012) Mouse and computational models link Mlc2v dephosphorylation to altered myosin kinetics in early cardiac disease. J. Clin. Invest. 122(4): 1209-1221. (*, Co-first author). Download this article

15. Fang H, Chen M, Ding Y, Shang W, Zhang X, Xu J, Zhang W, Li K, Xiao Y, Gao F, Shang S, Li J, Tian X, Wang S, Zhou J, Weisleder N, Ma J, Ouyang K, Chen J, Wang X, Zheng M, Wang W, Zhang X, Cheng H (2011) Imaging superoxide flash and metabolism-coupled mitochondrial permeability transition in living animals Cell Research 21: 1295-1304. Download this article

14. Hongqiang Cheng, Ming Zheng, Angela K. Peter, Kensuke Kimura, Xiaodong Li, Kunfu Ouyang, Tao Shen, Li Cui, Derk Frank, Nancy D. Dalton, Yusu Gu, Norbert Frey, Kirk L. Peterson, Sylvia M. Evans, Kirk U. Knowlton, Farah Sheikh, Ju Chen (2011) Selective deletion of long but not short Cypher isoforms leads to late onset dilated cardiomyopathy. Hum. Mol. Genet. 20: 1751-62. Download this article

13. Jem A. Efe, Simon Hilcove, Janghwan Kim, Hongyan Zhou, Kunfu Ouyang, Gang Wang, Ju Chen, Sheng Ding (2011) Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy. Nat. Cell Biol. 13: 215-222. Download this article

12. Guiling Zhao, Zachary Neeb, M. Dennis Leo, Judith Pachuau, Adebowale Adebiyi, Kunfu Ouyang, Ju Chen, Jonathan H. Jaggar (2010) Type 1 IP3 receptors activate BKCa channels via local molecular coupling in cerebral artery smooth muscle cells. J. Gen. Physiol. 136:283-291. Download this article

11. Hongqiang Cheng, Kensuke Kimura, Angela K. Peter, Li Cui, Kunfu Ouyang, Tao Shen, Yujie Liu, Yusu Gu, Nancy D. Dalton, Sylvia M. Evans, Kirk U. Knowlton, Kirk L. Peterson, Ju Chen (2010) Loss of Enigma homolog protein results in dilated cardiomyopathy. Circ. Res. 107: 348-356. Download this article

10. Chaoliang Wei, Xianhua Wang, Min Chen, Kunfu Ouyang, Ming Zheng, Heping Cheng (2010) Flickering calcium microdomains signal turning of migrating cells. Can. J. Physiol. Pharmacol. 88: 105-110 (Review). Download this article

9. Stephan Lange*, Kunfu Ouyang*, Gretchen Meyer, Li Cui, Hongqiang Cheng, Richard L. Lieber, Ju Chen (2009) Obscurin regulates longitudinal SR architecture. J. Cell. Sci. 122: 2640-2650 (*, Co-first Author). Download this article

8. Chaoliang Wei, Xianhua Wang, Min Chen, Kunfu Ouyang, Long-Sheng Song, Heping Cheng (2009) Calcium flickers steer cell migration. Nature 457: 901-905. Download this article

7. Ming Zheng, Hongqiang Cheng, Xiaodong Li, Jianlin Zhang, Li Cui, Kunfu Ouyang, Liang Han, Ting Zhao, Yusu Gu, Nancy D. Dalton, Marie-Louise Bang, Kirk L. Peterson, Ju Chen (2009) Cardiac-specific ablation of cypher leads to a severe form of dilated cardiomyopathy with premature death. Hum. Mol. Genet. 18: 701-713. Download this article

6. Wang Wang, Huaqiang Fang, Linda Groom, Aiwu Cheng, Wanrui Zhang, Jie Liu, Xianhua Wang, Kaitao Li, Peidong Han, Ming Zheng, Jinhu Yin, Weidong Wang, Mark P. Mattson, Joseph P. Y. Kao, Edward G. Lakatta, Shey-Shing Sheu, Kunfu Ouyang, Ju Chen, Robert T. Dirksen, Heping Cheng (2008) Superoxide Flashes in Single Mitochondria. Cell 134: 279-290. Download this article

5. Chen-Leng Cai, Jody C. Martin, Yunfu Sun, Li Cui, Liangchun Wang, Kunfu Ouyang, Lei Yang, Lei Bu, Xingqun Liang, Xiaoxue Zhang, Williang B. Stallcup, Christopher P. Denton, Andrew McCulloch, Ju Chen & Sylvia M. Evans (2008) A myocardial lineage derives from Tbx18 epicardial cells. Nature 454: 104-108. Download this article

4. Li-jun Yao, Gang Wang, Kun-fu Ou-Yang, Chao-liang Wei, Xian-hua Wang, Shi-rong Wang, Wei Yao, Hong-ping Huang, Jian-hong Luo, Cai-hong Wu, Jie Liu, Zhuan Zhou, He-ping Cheng (2006) Ca2+ sparks and Ca2+ glows in superior cervical ganglion neurons. Acta Pharmacol. Sin. 27: 848-852. Download this article

3. Kunfu Ouyang*, Hui Zheng*, Xiaomei Qin, Chen Zhang, Dongmei Yang, Xian Wang, Caihong Wu, Zhuan Zhou & Heping Cheng (2005) Ca2+ sparks and secretion in dorsal root ganglion neurons. Proc. Natl. Acad. Sci. USA 102: 12259-12264 (*, Co-first Author). Download this article

2. Kunfu Ouyang, Caihong Wu & Heping Cheng (2005) Ca2+-induced Ca2+ release in sensory neurons: low-gain amplification confers intrinsic stability. J. Biol. Chem. 280: 15898-15902. Download this article

1. Ben-De Zou, Kun-Fu Ouyang, Yu-Zhen Chen, Cai-Hong Wu & Pei-Ai Zhou (2001) Blockade of U50488H on potassium currents of acutely isolated mouse hippocampal CA3 pyramidal neurons. Brain Research 897: 52-59. Download this article